Membrane Detection with just simple edge detection

Today, I tried playing around with the membrane data set. I did the following

1. Created a difference of Gaussian image in GIMP
2. Enhanced the contrast in GIMP
3. Thresholded the image in MATLAB
4. Removed the connected components in the binary image that where less than
1. Specified Area
2. Major axis length
5. The overlay image was created.

The result of such a pipeline is shown below